Device

Part:BBa_K396006:Design

Designed by: Ling Li   Group: iGEM10_Chiba   (2010-10-21)


GFP pulse generator with pLux/cI434 hybrid promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 226
    Illegal XhoI site found at 325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

hybrid promoter has lux box in its upstream and cI434 operation site(OR1,OR2)in its downstream. The RBS of cI434 is weak and GFP is designed destabilized with LVA tag.


Source

Ron Weiss. Spatiotemporal control of gene expression with pulse-generating networks

References